Abstract:

This experiment was conducted to determine effects of molybdenum under stress of cadmium on red blood cell membrane antioxidation and XOD gene transcription of liver in goat.Thirty-six goats weighing approximately 20 kg each were randomly allocated into 4 groups of 9 goats each (three replicates every group).Goats of experiment group were drenched the same amount CdCl₂ and the different amount ［(NH₄)₆Mo₇O₂₄•4H₂O］，and goats of control group were drenched corresponding quantitative deionized water.Groups consisted of control group (0Cd+0Mo)，experiment groupⅠ(Cd 0.5 mg+Mo15 mg，per kg of body weight)，experiment group Ⅱ(Cd0.5 mg+Mo30 mg，per kg of body weight)，experiment group Ⅲ(Cd0.5 mg+Mo45 mg，per kg of body weight).Blood was collected on the 0th，10th，20th，30th，40th and 50th day and liver were collected on the 0th，25th and 50th day in experiment period from every group，then related indexes were determined.Results showed that XOD，LDH in red cell membrane of each experiment group were significantly raised，reduced ATPase，ALP activity were observed following the increase of experiment time，in addition，compared with control group，levels of XOD，LDH from each group were significantly higher while ATPase，ALP were lower on 50th day (P<0.01)，T-AOC had no statistical significance (P>0.05)；XOD transcription of liver in each experiment group showed a time-dependent effect，in addition，compared with control group，transcription of XOD significantly descend on 50th day (P<0.05).These results showed that red blood cell antioxidation decreased and XOD transcription of liver downregulated with the increasing of molybdenum under stress of cadmium in goats，and there was synergistic relationship between molybdenum and cadmium.